Journal: Biomaterials
Article Title: Shifts in macrophage phenotype at the biomaterial interface via IL-4 eluting coatings are associated with improved implant integration
doi: 10.1016/j.biomaterials.2016.10.019
Figure Lengend Snippet: (a) Picro Sirius Red stained tissue sections (20X) of mice implanted with a 1 cm2 piece of pristine, coated (no IL-4) and IL-4 loaded (40B) mesh at 90 days. Arrowheads indicate the capsule surrounding single mesh fibers. Scale bars represent 100 μm. (b) Image analysis of collagen capsule quality, surrounding mesh fibers (3 images of a single fiber at 20X per sample, N = 8). Bars represent the mean ± SEM. Statistical significance as (**) p < 0.01, (***) p < 0.001 and (****) p < 0.0001, using two-way ANOVA with Tukey's test. All other differences are non-significant.
Article Snippet: Picro Sirius Red custom-designed algorithm (MathWorks MathLab, VR2015a) was developed by Christopher Carruthers, Ph.D., Department of Bioengineering, University of Pittsburgh.
Techniques: Staining